Comparison of Various Precipitin Tests in the Detection of α-Fetoprotein in Serum
Megumi Nishioka, K. Okita, Yoshiaki Okamoto, Tetsuro Kodama, T Fujita
Abstract
Megumi Nishioka, K. Okita, Yoshiaki Okamoto, Tetsuro Kodama, T Fujita
Abstract
The Ouchterlony technique, single radial immunodiffusion and fluoro-electro-immunodiffusion (FEID) for the detection of α-fetoprotein (AFP) had their own merits. FEID was at least 30 times as sensitive as the Ouchterlony technique for the detection of serum AFP. Its great sensitivity elicited a positive reaction in sera which were previously negative by the Ouchterlony technique.
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The Ouchterlony technique, single radial immunodiffusion and fluoro-electro-immunodiffusion (FEID) for the detection of α-fetoprotein (AFP) had their own merits. FEID was at least 30 times as sensitive as the Ouchterlony technique for the detection of serum AFP. Its great sensitivity elicited a positive reaction in sera which were previously negative by the Ouchterlony technique.
Key concepts: Ouchterlony double immunodiffusion, Precipitin, Immunodiffusion, Radial immunodiffusion, Chemistry, Molecular biology, Biology, Immunology