Evaluation of Crude Extracts of Mimosa pusica LINN. against Colletotrichum gloeosporioides to Control Anthracnose

The antifungal activity of crude extracts of M. pudica Linn. was evaluated by determining the minimum inhibitory concentration (MIC) of the solvent extracts. Subsequently, an antifungal assay was carried out by determining the inhibition of radial growth in PDA plates added with plant extracts. MIC value of crude extracts was 15.00, 18.50 and 19.50 µg/mL for 95% ethanol, dichloromethane and acetone extracts, respectively. Concerning the inhibition of radial growth, ethanol crude extracts of M. Pudica was most effective and exhibited highest antifungal activity of 55.65% at 10.00 µg/mL, 54.02% at 1.00 µg/mL, 50.95% at 0.10 µg/mL, and 48.70% at 0.01 µg/mL against C. gloeosporioides., respectively. The highest yield was found in 95% ethanol at 73 mg/g. The higher yield was presented when the higher polar solvents were used in this experiment. Since factors such as solvent of extraction may influence the properties of derived crude extract, different extraction methods may be of interest for further trial.