Development and Validation of Derivative Spectrophotometric Method for Simultaneous Estimation of Levosulpiride and Esomeprazole in Capsule Dosage Form

The use of first order derivative spectrophotometry allows simultaneous determination of levosulpiride and esomeprazole in fixed dose combination products. The wavelengths 275 nm and 291.5 nm of first derivative spectrum were selected for the estimation of levosulpiride and esomeprazole, respectively without mutual interference. The method was linear in the concentration range 25–125 μg/ml and 5–25 μg/ml for levosulpiride and esomeprazole, respectively. The method was validated in terms of accuracy and precision. The result of the formulation analysis shows that the proposed method can be successfully used for the simultaneous estimation of both the drugs in their combined capsule dosage form.