Effect of Diet on Enzyme Profile, Biochemical Changes and In sacco Degradability of Feeds in the Rumen of Buffalo

METHODS Feeding of animals and experimental designFour rumen fistulated Murrah buffaloes of body weight ranging from 180-242 kg, were fed four diets consisting of roughage (wheat straw) and concentrate mixture in the ratios of 80:20, 70:30, 60:40 and 50:50 respectively, in a 4×4 Latin Square design.Wheat straw and concentrate mixture, consisting of wheat bran 37, crushed maize grain 30, mustard seed cake 30, mineral mixture 2 and salt 1 parts, were mixed with water (0.6 l/kg feed) manually and offered to animals as complete feed mixtures at 8:00 h and 16:00 h in two equal parts.Each feeding period lasted 30 days, and the sampling of rumen liquor and rumen contents was done after 25 days of feeding for two consecutive days.The rumen liquor was collected at 0, 2, 4, 6 and 8 h post feeding.After recording pH the samples were pooled for the day Effect of Diet on Enzyme Profile, Biochemical Changes and In sacco Degradability of Feeds in the Rumen of Buffalo