Evaluation of various cultural enrichment methods for the detection of selected food borne bacterial pathogens

Aim: The study was conducted to evaluate the performance of different enrichment broths such as Tryptic Soy Yeast extract Broth (TSBYE), Brain Heart Infusion broth (BHI), Nutrient Broth (NB), Luria Broth (LB) and Peptone water (PW) for the detection of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus and Salmonella enterica Typhimurium.Materials and Methods: The bacterial strains were procured from Institute of Microbial Technology (IMTECH), 0 0Chandigarh.Growth of these food borne pathogens at two different incubation temperatures (35 C and 37 C) and three different incubation periods (12h., 16h.and 18h.) were studied. Results:The result of the study showed that enrichment in Tryptic soy broth with yeast extract (TSBYE) and incubation at 0 37 C for 18h. is superior for the enrichment of all the organisms under study.Conclusion: TSBYE can be used very effectively as universal enrichment broth in comparison with all other enrichment broths studied for the detection of L. monocytogenes, Y. enterocolitica, S. aureus and S. enterica Typhimurium.