Control of root and shoot formation and production of trees from poplar callus

The technique of trees production from the undifferentiated poplar callus tissue is described. The best root formation was observed on the modified Wolter and Skoog medium when NAA in concentration 0.2 to 0.4 mg l −1 was used as an auxin and cytokinins were omitted. The induction of leafy shoots from the undifferentiated callus was the most effective on the modified Linsmaier and Skoog medium in the absence of auxin and with 0.15 to 0.70 mg l −1 of BAP. The best development of roots at the basal end of excised shoots was achieved when shoots were transferred into the sterile mixture of perlit and sand (3: l, v/v) containing a modified Wolter and Skoog medium.