Formation of Protoplasts from Strain Streptomyces Albogriseolus 444

Cultural conditions for protoplast preparation of strain Streptomyces albogriseolus 444, producing antibiotic complex with wide antimicrobial spectrum were studied. Effective formation and stabilization of the protoplasts was accomplished by 24 h cultivation of the strain 444 in a medium, containing 0.5% glycine and 1% sucrose. After that the mycelia were treated with 4 mg per ml lysozyme. Under these optimal conditions it amounted up to 5.13x107 protoplasts per ml.