Comparative analysis of lymphocyte phenotypes between carriers of human immunodeficiency virus(HIV) and adult patients with primary immunodeficiency using two-color immunofluorescence flow cytometry.

OHNO, T., KANOH, T., SUZUKI, T., MASUDA, T., KURIBAYASHI, K., ARAYA, S., ARAI, H. and UCHINO, H. Comparative Analysis of Lymphocyte Phenotypes between Carriers of Human Immunodeficiency Virus (HIV) and Adult Patients with Primary Immunodeficiency Using Two-Color Immunofluorescence Flow Cytometry Tohoku J. exp. Med., 1988, 154 (2), 157-172 - A variety of phenotypic abnormalities of peripheral blood lymphocytes from 8 HIV-carriers (HIVC), 6 patients with common variable Immunodeficiency disease (CVID), and 13 patients with selective immunoglobulin deficiency (SIgD) were compared using two-color flow cytometry. There was a close resemblance in phenotypic abnormalities between HIVC and the patients with CVID; i.e., increases in CD8+CD11-, Leu7+CD16- and CD3+DR+ cells, and decreases in CD4+Leu8+, CD4+Leu8-, Leu7+ CD16+ and Leu7-CD16+ cells. The increase in CD3+DR+ cells was due to an increase in CD8+DR+ cells. The CD4/CD8 ratio was inverted in both groups. A strong correlation coefficient (CC) was found only between the CD4/CD8 ratio and CD4+Leu8+ cells in HIVC, while CC was also high between the CD4/CD8 ratio and CD8+CD11- cells in CVID. The phenotypic abnormalities of the patients with SIgD were various and no significant difference was found against the control, except for an increase in CD4+Leu8+ cells and a decrease in CD4+Leu8- cells, which suggests heterogeneity of immunological deficits in this group. In severe immunodeficiency, ineffective killer cells appeared to be induced as a result of an adaptive change involved in recurrent or persistent viral infections, and Leu8 molecule may be concerned in the susceptibility of CD4+cells to HIV.