DIFFERENCES IN SEQUENCES OF RIBOSOMAL DNA SECOND INTERNAL TRANSCRIBED SPACER AMONG THREE MEMBERS OF ANOPHELES HYRCANUS COMPLEX FROM THE REPUBLIC OF KOREA

Abstract Differences in sequences of ribosomal DNA second internal transcribed spacer (ITS) among Anopheles sinensis, An. lesteri and An. yatsushiroensis from Korea were compared. The PCR amplified rDNA‐ITS2 fragments were sequenced directly. Three samples of each species were individually determined. Lengths of the ITS2 regions were 468bp in An sinensis, 451bp in An lesteri and 453bp in An yatsushiroenesis and GC contents were 44.87 % 46.2 % and 45.7 % respectively. Variations of the sequences ranged from 12.16 % to 30.7 % among the three species. The differences of the rDNA‐ITS2 sequences would be useful for molecular identification of the three members of Anopheles hycanus complex from Korea.