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1987•Journal of Liquid ChromatographyRequires access

Combined Florisil, Droplet Counter-Current, and High Performance Liquid Chromatographies for the Preparative Isolation and Purification of Azadirachtin from Neem (Azadirachta Indica) Seeds

S. Mark Lee, James A. Klocke

Open publisher page 8 citations

Abstract

An alternate prepurification procedure was developed for the isolation and purification of azadirachtin, a biologically active limonoid, from neem (Azadirachta indica) seeds by the use of Florisil and droplet counter-current chromatographies. The procedure, combined with the use of preparative high performance liquid chromatography, yielded 364.8 mg of >99% pure azadirachtin from 1.5 kg of Indian neem seeds.

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An alternate prepurification procedure was developed for the isolation and purification of azadirachtin, a biologically active limonoid, from neem (Azadirachta indica) seeds by the use of Florisil and droplet counter-current chromatographies. The procedure, combined with the use of preparative high performance liquid chromatography, yielded 364.8 mg of >99% pure azadirachtin from 1.5 kg of Indian neem seeds.

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Available abstract

An alternate prepurification procedure was developed for the isolation and purification of azadirachtin, a biologically active limonoid, from neem (Azadirachta indica) seeds by the use of Florisil and droplet counter-current chromatographies. The procedure, combined with the use of preparative high performance liquid chromatography, yielded 364.8 mg of >99% pure azadirachtin from 1.5 kg of Indian neem seeds.

Key concepts: Azadirachta, Azadirachtin, Limonoid, Chemistry, Meliaceae, Chromatography, Botany, Pesticide

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Combined Florisil, Droplet Counter-Current, and High Performance Liquid Chromatographies for the Preparative Isolation and Purification of Azadirachtin from Neem (Azadirachta Indica) Seeds — Research Paper | ScholarLens