1989•Journal of the Chemical Society Chemical CommunicationsRequires access
Sugar-responsible initiation–cessation control of enzyme release from gel matrix by use of an agarose/concanavalin A system
Etsuo Kokufuta, Isei Nakamura, Mitsuhiro Haraguchi, Akihiko Shimada
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Abstract
The diffusional release of exo-1,4-α-D-glucosidase from agarose gel stops when Concanavalin A binds to the gel, but its removal from the gel by D–mannose leads to rapid release; the reversible initiation–cessation control can be achieved in both batchwise and column operations by the alternate use of D-mannose and Concanavalin A.