2005•ASAIO JournalRequires access
HEMOLYSIS EVALUATION OF CENTRIFUGAL PUMP USING MICROCAPSULE SUSPENSION, BOVINE BLOOD, AND HUMAN BLOOD
Yoshio Ootaki, Keiji Kamohara, David J. Horvath, Stephan Benephit, Leonard A.R. Golding, Osamu Maruyama, Kiyotaka Fukamachi
Open publisher page 0 citations
Abstract
Purpose: Hemolysis is one of the most important test parameters for blood pumps. Bovine blood and human blood have been used for in vitro hemolysis testing. However, the results from bovine blood are complicated by the variations in individual RBC susceptibility to sheer. Also access to human blood is becoming increasingly difficult especially for testing blood pumps in the early stages of development. The purpose of this study was to evaluate microcapsule suspension as an alternative to bovine or human blood hemolysis testing. Methods: Six centrifugal pumps were used for hemolysis testing using microcapsule suspension, bovine blood, and human blood. Microcapsule suspension consists of 100 μm maximal diameter, polyurethane membrane capsules containing leuco dye dissolved in isopropyl naphthalene. Results: The hemolysis values using microcapsule suspension, bovine blood, and human blood correlated well with each other. Conclusions: Microcapsule suspension can be used to successfully screen blood pumps for hemolysis. The good correlation between human and calf hemolysis results suggests that human blood hemolysis levels can be predicted from bovine blood test data.Figure