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High Efficiency Transformation ofBacillus brevisby Electroporation

Akiko Okamoto, Akihiko Kosugi, Yukimichi KOIZUMI, Fujiharu YANAGIDA, Shigezo Udaka

Abstract

Various conditions in the Bacillus brevis transformation by electroporation were investigated to increase the efficiency. Competent cell volume and presence of polyethylene glycol 6000 and single stranded DNA on pulsing were critical for the efficiency. As a result, we established the improved method by which 10(7)-10(8) transformants/microgram plasmid DNA could be obtained.

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Available abstract

Key concepts: Electroporation, Transformation (genetics), Plasmid, Transformation efficiency, Polyethylene glycol, DNA, Bacillus (shape), Microbiology