Media for growth of flax tissue culture

A callus tissue culture from flax cotyledons reached maximum growth on a medium rich in mineral salts and supplemented with 2 mg/l α-naphthalene acetic acid, 0.05 mg/l kinetin, 15% coconut milk, and 3% sucrose. While tissue differentiation was evident and root formation was induced with increasing auxin concentrations, higher kinetin levels or the addition of L-tyrosine failed to promote shoot formation.