1984•Proceedings of the National Academy of SciencesOpen access
Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells.
Takashi Yokota, Frank Lee, Donna M. Rennick, Claire E. Hall, Naoko Arai, Tim R. Mosmann, Gary J. Nabel, Harvey Cantor, Ken‐ichi Arai
Open full text 356 citations
Abstract
A cDNA sequence coding for mouse mast-cell growth-factor (MCGF) has been cloned from a cDNA library prepared from mRNA derived from a concanavalin A-activated mouse T-cell clone. Cloning was achieved using the pcD vector that permits expression of cDNA inserts in mammalian cells. The DNA sequence codes for a polypeptide of 166 amino acid residues including a putative signal peptide. The supernatant fluid obtained after transfection of COS-7 monkey cells with the pcD-MCGF plasmid had mouse MCGF activity. The MCGF amino acid sequence between amino acids 33 and 41, deduced from the nucleotide sequence of its cDNA, is identical to the NH2-terminal sequence of the mouse lymphokine, interleukin 3, suggesting that MCGF is related to interleukin 3.