High performance liquid chromatography analyses of emulsifiers: I. Quantitative determinations of mono‐ and diacylglycerols of saturated fatty acids

Abstract A high performance liquid chromatography (HPLC) method is described for the separation of mono‐, di‐, and triacylglycerols of fatty acids on a 25‐cm column packed with 10 μm LiChrosorb DIOL. The acylglycerols are eluted isocratically with isooctane‐isopropanol (95:5) within 10 min, and the components monitored by UV‐absorption at 213 nm. The applicability of the method for the quantitative determination of the mono‐ and diacylglycerol content of fully hardened monoglycerides using an internal standard method has been demonstrated. The method shows excellent reproducibility and accuracy with standard deviations of a distilled monoglyceride containing 92.1% monoacylglycerols and 6.5% diacylglycerols of 0.70% and 0.42%, respectively. The method is applicable to other types of emulsifiers, for instance, acetylated monoglyceride emulsifiers and propylene glycol esters of fatty acids.