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Production of Monoclonal Antibodies Against Phosphoprotein 65 from Human Cytomegalovirus

Lijun Xu, Hangping Yao, Jun Fan, Dan Li, Nanping Wu

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Abstract

Human cytomegalovirus (HCMV) has a very high infection rate in the normal population. Early diagnosis is of great value, especially in immunosuppressed individuals. HCMV phosphoprotein 65 (pp65) is the most widely used antigen for diagnosis. In this report, we prepared a monoclonal antibody against pp65, which could be used for both immunohistochemistry and Western blot diagnosis of HCMV infection.

About this research paper

What this paper is about

Human cytomegalovirus (HCMV) has a very high infection rate in the normal population. Early diagnosis is of great value, especially in immunosuppressed individuals. HCMV phosphoprotein 65 (pp65) is the most widely used antigen for diagnosis. In this report, we prepared a monoclonal antibody against pp65, which could be used for both immunohistochemistry and Western blot diagnosis of HCMV infection.

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OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Human cytomegalovirus (HCMV) has a very high infection rate in the normal population. Early diagnosis is of great value, especially in immunosuppressed individuals. HCMV phosphoprotein 65 (pp65) is the most widely used antigen for diagnosis. In this report, we prepared a monoclonal antibody against pp65, which could be used for both immunohistochemistry and Western blot diagnosis of HCMV infection.

Key concepts: Phosphoprotein, Monoclonal antibody, Human cytomegalovirus, Immunohistochemistry, Virology, Cytomegalovirus, Western blot, Antigen

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