112 INCREASED SUBUROTHELIAL INFLAMMATION, INCREASED UROTHELIAL CELL APOPTOSIS AND DECREASED E-CADHERIN INTENSITY IN THE KETAMINE ASSOCIATED BLADDER DYSFUNCTION- AN IMMUNOHISTOCHEMISTRY COMPARISON WITH INTERSTITIAL CYSTITIS

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research II1 Apr 2012112 INCREASED SUBUROTHELIAL INFLAMMATION, INCREASED UROTHELIAL CELL APOPTOSIS AND DECREASED E-CADHERIN INTENSITY IN THE KETAMINE ASSOCIATED BLADDER DYSFUNCTION- AN IMMUNOHISTOCHEMISTRY COMPARISON WITH INTERSTITIAL CYSTITIS Cheng-Ling Lee, Jia-Heng Shie, Yue-Shiung Wang, Shiu-Dong Chung, and Hann-Chorng Kuo Cheng-Ling LeeCheng-Ling Lee Hualien, Taiwan More articles by this author , Jia-Heng ShieJia-Heng Shie Hualien, Taiwan More articles by this author , Yue-Shiung WangYue-Shiung Wang Hualien, Taiwan More articles by this author , Shiu-Dong ChungShiu-Dong Chung Taipei, Taiwan More articles by this author , and Hann-Chorng KuoHann-Chorng Kuo Hualien, Taiwan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.160AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Altered urothelial homeostasis and activation of mast cells have been found to be the possible causes of interstitial cystitis/painful bladder syndrome (IC/PBS). Ketamine is an emergent drug abused by Asian youth as a drug for pleasure. The bladder dysfunction in ketamine abuser presents a more severe form of end-stage bladder manifested with contracted bladder, bilateral ureteral obstruction, easy bladder bleeding, frequent urinary tract infection and severe bladder pain. This study investigated the suburothelial inflammation and urothelial dysfunction between ketamine cystitis (KC) and IC/PBS and try to explore the underlying pathophysiology of KC. METHODS Bladder tissues from 11 KC, 17 IC/PBS, and 10 controls were included in this study. Immunofluorescence (IF) staining of junction protein E-cadherin, tryptase (mast cell activation) and TUNEL (urothelial cell apoptosis) were performed. The fluorescence intensity of E-cadherin was measured using an Image J method. The percentage of apoptotic cells and activated mast cells were measured and quantified as positive cell per area unit (4 μm2). RESULTS The mean age was 24.9±3.7, 41.3±13.7, and 50.5±9.6 years old in KC, IC/PBS and controls, respectively (all p<0.05). TUNEL staining revealed a significant increase of apoptotic cells in IC/PBS (2.42±1.75) compared to controls (0.08±0.26) and further increased in KC (4.18±1.46) (all comparison, p=0.000). The tryptase signal was also significantly increased in IC/PBS (4.64±2.96) compared to controls (1.25±1.15) and was highest in KC (7.51±3.63), (all p=0.000). IF also showed significantly reduced distribution of E-cadherin in KC (10.1±11.2), IC/PBS (25.1±16.3) compared to controls (42.4±16.7) (all p<0.05). Increased apoptotic cells and decreased expression of E-cadherin were significantly correlated with increased visual analog pain score and increased mast cell activity in overall bladders (p<0.05). CONCLUSIONS Increased suburothelial inflammation, increased urothelial cell apoptosis and defective junctional protein E-cadherin expression were more severe in KC bladders than IC/PBS. These findings correlated with clinical symptoms between KC and IC/PBS. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e45-e46 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Cheng-Ling Lee Hualien, Taiwan More articles by this author Jia-Heng Shie Hualien, Taiwan More articles by this author Yue-Shiung Wang Hualien, Taiwan More articles by this author Shiu-Dong Chung Taipei, Taiwan More articles by this author Hann-Chorng Kuo Hualien, Taiwan More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...