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1981•European Journal of BiochemistryRequires access

Stability of Non‐polyadenylated Viral mRNAs Injected into Frog Qucytes

Malcolm A. McCrae, Hugh R. Woodland

Open publisher page 26 citations

Abstract

The naturally non-polyadenylated mRNAs of reovirus were shown to have a half-life in excess of 3 days when injected into Xenopus laevis oocytes. Hybridization analysis and gel electrophoresis showed that all 10 mRNA species had a similar high stability, despite being translated at widely differing rates. We have confirmed previous findings indicating a role for the 5'-terminal cap structure in determining reovirus mRNA stability [Furuichi et al. (1977) Nature (Lond.) 266, 235-239]. The significance of these results in relation to a general role for poly(A) in messenger RNA function is discussed.

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The naturally non-polyadenylated mRNAs of reovirus were shown to have a half-life in excess of 3 days when injected into Xenopus laevis oocytes. Hybridization analysis and gel electrophoresis showed that all 10 mRNA species had a similar high stability, despite being translated at widely differing rates. We have confirmed previous findings indicating a role for the 5'-terminal cap structure in determining reovirus mRNA stability [Furuichi et al. (1977) Nature (Lond.) 266, 235-239]. The significance of these results in relation to a general role for poly(A) in messenger RNA function is discussed.

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Available abstract

The naturally non-polyadenylated mRNAs of reovirus were shown to have a half-life in excess of 3 days when injected into Xenopus laevis oocytes. Hybridization analysis and gel electrophoresis showed that all 10 mRNA species had a similar high stability, despite being translated at widely differing rates. We have confirmed previous findings indicating a role for the 5'-terminal cap structure in determining reovirus mRNA stability [Furuichi et al. (1977) Nature (Lond.) 266, 235-239]. The significance of these results in relation to a general role for poly(A) in messenger RNA function is discussed.

Key concepts: Polyadenylation, Xenopus, Messenger RNA, RNA, Biology, Molecular biology, Cell biology, Genetics

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