1994•Journal of ParasitologyRequires access

Improvement of the Merthiolate-Iodine-Formalin (MIF) Fecal Technique for Hookworm, Trichuris trichiura, and Ascaris lumbricoides Eggs

Monte P. Bawden

Abstract

The merthiolate-iodine-formalin (MIF) technique to detect human intestinal parasites in feces was inefficient for the detection of hookworm and Trichuris trichiura eggs. Addition of a saturated-brine flotation step increased recovery of hookworm eggs by 77% and Trichuris trichiura eggs by 87%. Recovery of Ascaris lumbricoides also was increased by 71%. This was a significant improvement in the sensitivity of the MIF technique for these 3 kinds of human parasite.

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Key concepts: Ascaris lumbricoides, Trichuris trichiura, Trichuris, Biology, Trichuriasis, Feces, Hookworm infection, Helminths