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1970•Journal of Cellular PhysiologyRequires access

The use of pronase in tissue culture: A comparison with trypsin

John F. Foley, Byron Th. Aftonomos

Open publisher page 27 citations

Abstract

Abstract Pronase is a proteolytic enzyme recently introduced as an effective dispersing agent of tissue culture cells. We have found it to be a most rapid and complete dispersing agent for primary fibroblastic cell lines and clearly superior to trypsin. On the other hand, with certain continuous epithelial cell lines its completeness of dispersion is inferior to that of trypsin necessitating study of each cell line individually with both enzymes to select the most advantageous one.

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What this paper is about

Abstract Pronase is a proteolytic enzyme recently introduced as an effective dispersing agent of tissue culture cells. We have found it to be a most rapid and complete dispersing agent for primary fibroblastic cell lines and clearly superior to trypsin. On the other hand, with certain continuous epithelial cell lines its completeness of dispersion is inferior to that of trypsin necessitating study of each cell line individually with both enzymes to select the most advantageous one.

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Available abstract

Abstract Pronase is a proteolytic enzyme recently introduced as an effective dispersing agent of tissue culture cells. We have found it to be a most rapid and complete dispersing agent for primary fibroblastic cell lines and clearly superior to trypsin. On the other hand, with certain continuous epithelial cell lines its completeness of dispersion is inferior to that of trypsin necessitating study of each cell line individually with both enzymes to select the most advantageous one.

Key concepts: Pronase, Trypsin, Cell culture, Proteolytic enzymes, Enzyme, Biochemistry, Tissue culture, Cell

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