2014•Journal of Structural BiologyRequires access

Robust and low cost uniform 15N-labeling of proteins expressed in Drosophila S2 cells and Spodoptera frugiperda Sf9 cells for NMR applications

Annalisa Meola, Célia Deville, Scott A. Jeffers, Pablo Guardado‐Calvo, Ieva Vasiliauskaité-Brooks, Christina Sizun, Christine Girard-Blanc, Christian Malosse, Carine van Heijenoort, Julia Chamot‐Rooke, Thomas Krey, Éric Guittet, Stéphane Pêtres, Felix Augusto Rey, François Bontems

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Key concepts: Sf9, Spodoptera, Biology, Escherichia coli, Biochemistry, Stable isotope labeling by amino acids in cell culture, Schneider 2 cells, Nuclear magnetic resonance spectroscopy