Andreas Babaryka, Enrico Kühn, Reinhard Wolfgang Köster
Abstract
The authors show that co-injection at the one-cell stage of mRNA encoding a nuclear-targeted meganuclease I-SceI together with expression cassettes flanked by cognate restriction sites results in efficient stable transgenesis in zebrafish Danio rerio.
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The authors show that co-injection at the one-cell stage of mRNA encoding a nuclear-targeted meganuclease I-SceI together with expression cassettes flanked by cognate restriction sites results in efficient stable transgenesis in zebrafish Danio rerio.
Key concepts: Danio, Biology, Zebrafish, Transgene, Zoology, In vivo, Genetics, Evolutionary biology