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ESTIMATION OF LIPASE IN DAIRY PRODUCTS: I. AN EXTRACTION-TITRATION METHOD FOR THE ESTIMATION OF MILK LIPASE

L.M. Smith, D. J. Lubert, H. R. Thornton

Open publisher page 9 citations

Abstract

A procedure has been outlined for the estimation of milk lipase. The method is based on the titration of an ether extract of butyric acid derived from the enzymatic hydrolysis of tributyrin under standardized conditions. Some of the fundamental factors involved in the procedure have been discussed. It is suggested that modifications of the method may be used to study lipase activity in other dairy products and in cultures of micro-organisms.

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What this paper is about

A procedure has been outlined for the estimation of milk lipase. The method is based on the titration of an ether extract of butyric acid derived from the enzymatic hydrolysis of tributyrin under standardized conditions. Some of the fundamental factors involved in the procedure have been discussed. It is suggested that modifications of the method may be used to study lipase activity in other dairy products and in cultures of micro-organisms.

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OpenAlex reports 9 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

A procedure has been outlined for the estimation of milk lipase. The method is based on the titration of an ether extract of butyric acid derived from the enzymatic hydrolysis of tributyrin under standardized conditions. Some of the fundamental factors involved in the procedure have been discussed. It is suggested that modifications of the method may be used to study lipase activity in other dairy products and in cultures of micro-organisms.

Key concepts: Tributyrin, Lipase, Titration, Chromatography, Triacylglycerol lipase, Hydrolysis, Chemistry, Extraction (chemistry)

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ESTIMATION OF LIPASE IN DAIRY PRODUCTS: I. AN EXTRACTION-TITRATION METHOD FOR THE ESTIMATION OF MILK LIPASE — Research Paper | ScholarLens