Polyhydroxyalkanoate (PHA) Synthesis by Class IV PHA Synthases Employing Ralstonia eutropha PHB − 4 as Host Strain
Manami Hyakutake, Yuta Saito, Satoshi Tomizawa, Kouhei Mizuno, Takeharu Tsuge
Abstract
Manami Hyakutake, Yuta Saito, Satoshi Tomizawa, Kouhei Mizuno, Takeharu Tsuge
Abstract
Class IV polyhydroxyalkanoate (PHA) synthase from Bacillus cereus YB-4 (PhaRC(YB4)) or B. megaterium NBRC15308(T) (PhaRC(Bm)) was expressed in Ralstonia eutropha PHB(-)4 to compare the ability to produce PHA and the substrate specificity of PhaRCs. PhaRC(YB4) produced significant amounts of PHA and had broader substrate specificity than PhaRC(Bm).
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Class IV polyhydroxyalkanoate (PHA) synthase from Bacillus cereus YB-4 (PhaRC(YB4)) or B. megaterium NBRC15308(T) (PhaRC(Bm)) was expressed in Ralstonia eutropha PHB(-)4 to compare the ability to produce PHA and the substrate specificity of PhaRCs. PhaRC(YB4) produced significant amounts of PHA and had broader substrate specificity than PhaRC(Bm).
Key concepts: Ralstonia, Polyhydroxyalkanoates, Strain (injury), Cupriavidus necator, Host (biology), Microbiology, Chemistry, Bioplastic