Inhibition of Saccharomyces Cerevisiae by Combination of Hydrostatic Pressure and Monoterpenes

ABSTRACT Using a pressure of 1800 kg/cm 2 and 150 μg/mL of α‐terpinene for 1 hr at 25°C, growth reduction of 6 log cycles after incubation at 30°C/24h, was found with S. cerevisiae. Without pressurization, S. cerevisiae was resistant to 300 μg/mL. and 600 μg/mL α‐terpinene but was sensitive 1250 μg/mL. Reduction of 3 log cycles in viable cell counts was found when cells were exposed to pressure (1800 kg/cm 2 ) and ‐(+)‐limonene (200, 400 and 2,000 μg/mL).