2012•Chemical CommunicationsRequires access
Pyrosequencing-based barcodes for a dye-free multiplex bioassay
Zhiyao Chen, Xiaoying Fu, Xiaodan Zhang, Xiqun Liu, Bingjie Zou, Haiping Wu, Qinxin Song, Jin‐Heng Li, Tomoharu Kajiyama, Hideki Kambara, Guohua Zhou
Open publisher page 13 citations
Abstract
A novel dye-free labeling method for a multiplex bioassay was proposed by using short sequence-based barcodes consisting of a reporter base and repeats of two stuffer bases; then, the barcodes were quantitatively decoded by a single pyrosequencing assay without any pre-separation.