P0394 ROLE OF ANTI-TRANSGLUTAMINASE ANTIBODIES IN THE DIAGNOSIS OF CHILDREN COALIAC DISEASE: RESULTS OF A FIVE YEARS PROSPECTIVE STUDY

Introduction: The aim of this study is to evaluate the interest of IgA antibodies to tissue transglutaminase in the diagnosis of children celiac disease compared with anti-endomysium and anti-gliadin antibodies. Methods: Seventy one children with coeliac disease (mean age: 5 years and 8 months) and 101 disease controls (mean age: 4 years and 5 months). IgA anti-transglutaminase were tested by ELISA using a human recombinant tissue transglutaminase. IgA anti endomysium were detected by indirect immunofluorescence on monkey oesophagus. Results: The middle rate of IgA anti-transglutaminase was 101,06 AU in patients and only 0,47 AU in controls. IgA anti-transglutaminase and IgA anti-endomysium were in agreement in 98,8 % of cases; only 2 cases were discordant (+/− and −/+). Globally, the 2 markers had the same sensitivity (90,14 %), specificity (98,02 %), negative (93,39 %) and positive (96,97 %) predictive values. For anti-gliadin antibodies, the IgG were more sensitive (88,73 %) and the IgA more specific (93,39 %). Conclusion: IgA anti-tissue transglutaminase can be used instead of IgA anti-endomysium as a serological marker of screening and diagnosis of coeliac disease.