2004•FEBS LettersOpen access
TNF‐α and IL‐4 regulate expression of fractalkine (CX3CL1) as a membrane‐anchored proadhesive protein and soluble chemotactic peptide on human fibroblasts
Mamoru Yoshikawa, Toshiharu Nakajima, Kenji Matsumoto, Naoko Okada, Toshiharu Tsukidate, Makoto Iida, Nobuyoshi Otori, Shinichi Haruna, Hiroshi Moriyama, Toshio Imai, Hirohisa Saito
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Abstract
The CX(3)C chemokine, fractalkine (FKN, CX(3)CL1), has multiple functions and exists as two distinct forms, a membrane-anchored protein and a soluble chemotactic peptide that cleaves from the cell surface FKN. In this study, we first demonstrated the expression of FKN in tumor necrosis factor (TNF)-alpha- and interleukin (IL)-4-stimulated human fibroblasts. The induction of FKN was observed for both forms. We also demonstrated monocyte chemotactic activity in the culture supernatant from the fibroblasts stimulated with these cytokines. These results suggest that TNF-alpha- and IL-4-stimulated fibroblasts may play an important role in accumulation of monocytes at inflammatory sites.
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