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2009•Research in MicrobiologyOpen access

Differential immunoproteomics enables identification of Metarhizium anisopliae proteins related to Rhipicephalus microplus infection

Lucélia Santi, Walter O. Beys‐da‐Silva, Antônio F. M. Pinto, Augusto Schrank, Marilene Henning Vainstein

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Abstract

Differential immunoproteomics was applied to identify proteins secreted by Metarhizium anisopliae induced by the Rhipicephalus microplus cuticle. In addition, IgG anti-spore surface proteins were used for searching for proteins possibly involved in early stages of fungus versus tick infection. LC-MS/MS of differentially secreted proteins led to the identification of proteases (carboxypeptidase and Pr1A), chitinase, carboxylic acid transport and proline-rich protein. Differential immunoproteomics strategy facilitated the detection and the identification of new proteins related to M. anisopliae host-pathogen interaction and could be used in further works to identify novel proteins related to other microbial infection systems.

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What this paper is about

Differential immunoproteomics was applied to identify proteins secreted by Metarhizium anisopliae induced by the Rhipicephalus microplus cuticle. In addition, IgG anti-spore surface proteins were used for searching for proteins possibly involved in early stages of fungus versus tick infection. LC-MS/MS of differentially secreted proteins led to the identification of proteases (carboxypeptidase and Pr1A), chitinase, carboxylic acid transport and proline-rich protein. Differential immunoproteomics strategy facilitated the detection and the identification of new proteins related to M. anisopliae host-pathogen interaction and could be used in further works to identify novel proteins related to other microbial infection systems.

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Available abstract

Differential immunoproteomics was applied to identify proteins secreted by Metarhizium anisopliae induced by the Rhipicephalus microplus cuticle. In addition, IgG anti-spore surface proteins were used for searching for proteins possibly involved in early stages of fungus versus tick infection. LC-MS/MS of differentially secreted proteins led to the identification of proteases (carboxypeptidase and Pr1A), chitinase, carboxylic acid transport and proline-rich protein. Differential immunoproteomics strategy facilitated the detection and the identification of new proteins related to M. anisopliae host-pathogen interaction and could be used in further works to identify novel proteins related to other microbial infection systems.

Key concepts: Metarhizium anisopliae, Biology, Rhipicephalus microplus, Metarhizium, Microbiology, Proteases, Entomopathogenic fungus, Chitinase

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Differential immunoproteomics enables identification of Metarhizium anisopliae proteins related to Rhipicephalus microplus infection — Research Paper | ScholarLens