2013•Frontiers in Neural CircuitsOpen access
Next-generation transgenic mice for optogenetic analysis of neural circuits
Brent Asrican, George J Augustine, Ken Berglund, Susu Chen, Nick Chow, Karl Deisseroth, Guoping Feng, Bernd Gloss, Riichiro Hira, Carolin Hoffmann, Haruo Kasai, Malvika Katarya, Jinsook Kim, John Kudolo, Li Ming Lee, Shun Qiang Lo, James J. Mancuso, M Matsuzaki, Ryuichi Nakajima, Li Qiu, Gregory Tan, Yanxia Tang, Jonathan T. Ting, Sachiko Tsuda, Lei Wen, Xuying Zhang, Shengli Zhao
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Abstract
Here we characterize several new lines of transgenic mice useful for optogenetic analysis of brain circuit function. These mice express optogenetic probes, such as enhanced halorhodopsin or several different versions of channelrhodopsins, behind various neuron-specific promoters. These mice permit photoinhibition or photostimulation both in vitro and in vivo. Our results also reveal the important influence of fluorescent tags on optogenetic probe expression and function in transgenic mice.
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