1978•Analytical LettersRequires access

Quantitative Analysts of 5-Hydroxytryptamtne in Biological Material by High Perfornance Liquid Chromatography and Electrochmical Detection

Wchrister Hansson, Evald Rosengren

Abstract

A quantitative method for the analysis of 5-hydroxytryptamine in biological material is described. The method is based on high performance liquid chromatography (HPLC) with electrochemical detection. A simple purification on a weakly acidic ion exchange resin prior to the analysis gives quite clean samples and permits concentration of diluted samples. The chromatographic separation is performed on a reverse phase column with organic modifier added to an aqueous eluent. With this analytical system 25 pg of 5-hydroxytryptamine can be detected.

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Key concepts: Chemistry, Chromatography, High-performance liquid chromatography, Column chromatography, Quantitative analysis (chemistry), Aqueous solution, Ion chromatography, Reversed-phase chromatography