2007•Proceedings of the National Academy of SciencesOpen access
Extensively cross-reactive anti-HIV-1 neutralizing antibodies induced by gp140 immunization
Pengfei Zhang, Fatim Cham, Ming Chui Dong, Anil Choudhary, Peter Bouma, Zhiqiang Zhang, Yiming Shao, Yanru Feng, Lemin Wang, Nathalie Mathy, Gérald Voss, Christopher C. Broder, Gerald V. Quinnan
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Abstract
An immunization regimen was evaluated in rabbits consisting of the soluble, oligomeric form of envelope glycoprotein of HIV-1, strain R2 (gp140(R2)), or the surface component of the same envelope (Env), gp120(R2), in the adjuvant AS02A. The gp140(R2) was selected based on its unusual CD4-independent phenotype and the exceptionally broad neutralizing response in the infected donor. The gp140(R2) immunogen induced antibodies that achieved 50% neutralization of 48/48, and 80% neutralization of 43/46 primary strains of diverse HIV-1 subtypes tested. The strains tested included members of standard panels of subtype B and C strains, and other diverse strains known to be neutralization resistant. The gp120(R2) induced antibodies that neutralized 9/48 of the same strains. Neutralization was IgG-mediated and HIV-1-specific. These results demonstrate that induction of truly broad spectrum neutralizing antibodies is an achievable goal in HIV-1 vaccine development.
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