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2000•Bioscience Biotechnology and BiochemistryOpen access

Tributyrin Specifically Induces a Lipase with a Preference for the sn-2 Position of Triglyceride in Geotrichum sp. FO401B

Yasuhide Ota, Takeshi SAWAMOTO, Masaki Hasuo

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Abstract

The extracellular Lipases A and C produced by Geotrichum sp. FO401B have a preference for the sn-1,3 and sn-2 positions of triglyceride, respectively. Total production of these lipases was increased by plant oils and tributyrin. Butyl Toyopearl column chromatography demonstrated that only Lipase C was produced in the presence of tributyrin. Lipase C hydrolysed natural fats except sardine oil preferentially at the sn-2 position, but it showed little stereoselectivity for triolein.

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The extracellular Lipases A and C produced by Geotrichum sp. FO401B have a preference for the sn-1,3 and sn-2 positions of triglyceride, respectively. Total production of these lipases was increased by plant oils and tributyrin. Butyl Toyopearl column chromatography demonstrated that only Lipase C was produced in the presence of tributyrin. Lipase C hydrolysed natural fats except sardine oil preferentially at the sn-2 position, but it showed little stereoselectivity for triolein.

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Available abstract

The extracellular Lipases A and C produced by Geotrichum sp. FO401B have a preference for the sn-1,3 and sn-2 positions of triglyceride, respectively. Total production of these lipases was increased by plant oils and tributyrin. Butyl Toyopearl column chromatography demonstrated that only Lipase C was produced in the presence of tributyrin. Lipase C hydrolysed natural fats except sardine oil preferentially at the sn-2 position, but it showed little stereoselectivity for triolein.

Key concepts: Tributyrin, Lipase, Triolein, Geotrichum, Triglyceride, Chemistry, Hydrolysis, Triacylglycerol lipase

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Tributyrin Specifically Induces a Lipase with a Preference for the sn-2 Position of Triglyceride in Geotrichum sp. FO401B — Research Paper | ScholarLens