Validation of a High Performance Liquid Chromatography (HPLC) Method to Detect and Quantify Some Antibiotics Residues in Honey

Honey is a natural product with numerous beneficial effects in human nutrition and medicine.However it may be contaminated with substances hazardous to the consumers such as antibiotics which are frequently used to treat bee colonies or to promote their growth.In this study we firstly optimized and validated the detection parameters of chloramphenicol and streptomycin in honey by High Performance Liquid Chromatography (HPLC), and then we conducted experiments to adopt a method of extraction of theses antibiotics residues from honey.The validation of our analytical method required the setting of multiples statistique tests.The coefficient of variation (CV) calculated for streptomycin and chloramphenicol is 1.89% and 3.85% respectively, indicating a good repetitiveness of our analytical methods.To evaluate reproducibility, we use Student Test at 5 % risk degree, the calculated "t" is less than the "t" table in the three sets of comparison (2.09).A good correlation between concentration and the response of the detector is attested by a correlation coefficient "r" equal to 0.99 for chloramphenicol and 0.93 for streptomycin.The detection limit determined for streptomycin was 0.06 ppb and 0.024 ppm for chloramphenicol while limits of quantification are 0.2 ppb for Streptomycin and 0.08 ppm for Chloramphenicol.These results will be used later to identify and quantify these antibiotics residues in a large range of samples.