2005•Analytical BiochemistryRequires access

Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis

Paul Monis, Steven Giglio, Christopher P. Saint

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Key concepts: Melting curve analysis, SYBR Green I, Amplicon, Real-time polymerase chain reaction, Polymerase chain reaction, Intercalation (chemistry), Chemistry, Standard curve