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Behavior of Inc-Q plasmids in Agrobacterium tumefaciens

Jacques Hille, R. A. Schilperoort

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Abstract

Inc -Q plasmids were introduced into Agrobacterium tumefaciens , by mobilization from Escherichia coli with an Inc -P plasmid, or by transformation with purified plasmid DNA. It was found that they were stably maintained. The presence of an Inc -Q plasmid did not influence tumorigenicity. These results suggest that these plasmids may be used in genetic complementation studies of Ti plasmid mutants in A. tumefaciens .

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Inc -Q plasmids were introduced into Agrobacterium tumefaciens , by mobilization from Escherichia coli with an Inc -P plasmid, or by transformation with purified plasmid DNA. It was found that they were stably maintained. The presence of an Inc -Q plasmid did not influence tumorigenicity. These results suggest that these plasmids may be used in genetic complementation studies of Ti plasmid mutants in A. tumefaciens .

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Available abstract

Inc -Q plasmids were introduced into Agrobacterium tumefaciens , by mobilization from Escherichia coli with an Inc -P plasmid, or by transformation with purified plasmid DNA. It was found that they were stably maintained. The presence of an Inc -Q plasmid did not influence tumorigenicity. These results suggest that these plasmids may be used in genetic complementation studies of Ti plasmid mutants in A. tumefaciens .

Key concepts: Agrobacterium tumefaciens, Plasmid, T-DNA Binary system, Biology, Ti plasmid, Complementation, Transformation (genetics), Genetics

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