2006•Biocatalysis and BiotransformationRequires access

Isomaltose formation by free and immobilized dextransucrase

Sonja Berensmeier, Hans‐Joachim Jördening, Klaus Buchholz

Abstract

The acceptor reaction of dextransucrase from Leuconostoc mesenteroides NRRL-B512F with glucose as acceptor is of technical interest for isomaltooligosaccharide (IMOs) synthesis. Different experimental conditions were investigated for free and immobilized enzyme. The data for oligosaccharide formation up to a degree of polymerization 4 were correlated with a model developed earlier, and optimal reaction conditions for immobilized dextransucrase design and application were identified for later continuous application. Furthermore, stability was investigated for free and immobilized enzyme including stabilization by sugars.

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Available abstract

Key concepts: Dextransucrase, Chemistry, Leuconostoc mesenteroides, Isomaltose, Immobilized enzyme, Oligosaccharide, Enzyme, Glucosyltransferases