1979Australian Journal of BotanyRequires access

Some Conditions Governing Zoospore Production in Axenic Cultures of Phytophthora cinnamomi Rands

P. N. Byrt, BR Grant

Open publisher page 84 citations

Abstract

A method for the production of zoospores from the plant pathogen Phytophthora cinnamomi is described. The use of siliconized cellulose pads as a hyphal support, which in turn allows shake- grown mycelia to be used, results in the production of zoospores in concentrations of 107-108/litre. It allows one person to produce the equivalent of 1-10 mg of zoospore protein per batch, which is sufficient for a variety of biochemical studies.

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What this paper is about

A method for the production of zoospores from the plant pathogen Phytophthora cinnamomi is described. The use of siliconized cellulose pads as a hyphal support, which in turn allows shake- grown mycelia to be used, results in the production of zoospores in concentrations of 107-108/litre. It allows one person to produce the equivalent of 1-10 mg of zoospore protein per batch, which is sufficient for a variety of biochemical studies.

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Available abstract

A method for the production of zoospores from the plant pathogen Phytophthora cinnamomi is described. The use of siliconized cellulose pads as a hyphal support, which in turn allows shake- grown mycelia to be used, results in the production of zoospores in concentrations of 107-108/litre. It allows one person to produce the equivalent of 1-10 mg of zoospore protein per batch, which is sufficient for a variety of biochemical studies.

Key concepts: Zoospore, Phytophthora cinnamomi, Axenic, Biology, Mycelium, Phytophthora, Botany, Hypha

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