Some Conditions Governing Zoospore Production in Axenic Cultures of Phytophthora cinnamomi Rands
P. N. Byrt, BR Grant
Abstract
P. N. Byrt, BR Grant
Abstract
A method for the production of zoospores from the plant pathogen Phytophthora cinnamomi is described. The use of siliconized cellulose pads as a hyphal support, which in turn allows shake- grown mycelia to be used, results in the production of zoospores in concentrations of 107-108/litre. It allows one person to produce the equivalent of 1-10 mg of zoospore protein per batch, which is sufficient for a variety of biochemical studies.
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A method for the production of zoospores from the plant pathogen Phytophthora cinnamomi is described. The use of siliconized cellulose pads as a hyphal support, which in turn allows shake- grown mycelia to be used, results in the production of zoospores in concentrations of 107-108/litre. It allows one person to produce the equivalent of 1-10 mg of zoospore protein per batch, which is sufficient for a variety of biochemical studies.
Key concepts: Zoospore, Phytophthora cinnamomi, Axenic, Biology, Mycelium, Phytophthora, Botany, Hypha