1967•Canadian Journal of BiochemistryRequires access

PARTICIPATION OF NICOTINAMIDE MONONUCLEOTIDE IN PYRIDINE NUCLEOTIDE SYNTHESIS IN TUMOR AND HOST LIVER

J.W.D. McDonald, H. B. Stewart

Abstract

Evidence is presented to show that nicotinamide-7-14C in low or high doses is incorporated into nicotinamide mononucleotide (NMN) by Ehrlich ascites cells and host liver in vivo. Nicotinamide mononucleotide formed from nicotinamide-7-14C has been isolated from incubations of Sephadex-treated extracts of ascites cell acetone powders with 5-phosphoribosyl-1-pyrophosphate and characterized by spectral and chemical analysis. The apparent Kmand Vmaxfor NMN synthesis from nicotinamide have been determined. NMN-14C has been shown to form NAD-14C in the same extracts in the presence of ATP, and the apparent Vmaxand Kmof this process have been determined. The role of NMN in NAD synthesis is discussed.

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Key concepts: Nicotinamide mononucleotide, Nicotinamide, NAD+ kinase, Pyrophosphate, Chemistry, Sephadex, Nucleotide, Biochemistry