1991•Journal of Liquid ChromatographyRequires access

High-Performance Liquid Chromatographic Determination of Fusilade Using a Fluorescence Reagent, 4-Bromomethyl-7-Methoxycoumarin

Huguette Cohen, Bernadette Boutin-muma

Abstract

A high-performance liquid chromatography method for Fusilade has been developed which utilizes 4-bromomethyl 1-7-methoxycoumarin to provide derivative for high-sensitivity detection. the reagent reacts with Fusilade to form a fluorescent derivative which is separated on a RP-18 column (5 um) anddetected at ex: 325 nm, em: 395 nm. Fusilade can be determined in the range of at least 1 nmol, with the detection limit at 0.5 ng.

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Key concepts: Reagent, Detection limit, Chromatography, Derivative (finance), Fluorescence, Chemistry, High-performance liquid chromatography, Linear range