One‐Pot Cascade Reactions using Fructose‐6‐phosphate Aldolase: Efficient Synthesis of D ‐Arabinose 5‐Phosphate, D ‐Fructose 6‐Phosphate and Analogues

Abstract One‐pot multienzymatic reactions have been performed for the synthesis of 1‐deoxy‐D‐fructose 6‐phosphate, 1,2‐dideoxy‐D‐arabino‐hept‐3‐ulose 7‐phosphate, D‐fructose 6‐phosphate and D‐arabinose 5‐phosphate. The whole synthetic strategy is based on an aldol addition reaction catalysed by fructose‐6‐phosphate aldolase (FSA) as a key step of a three or four enzymes‐catalysed cascade reaction. The four known donors for FSA – dihydroxyacetone (DHA), hydroxyacetone (HA), 1‐hydroxy‐2‐butanone (HB) and glycolaldehyde (GA) – were used with D‐glyceraldehyde 3‐phosphate as acceptor substrate. The target phosphorylated sugars were obtained in good to excellent yields and high purity.