Synergistic effect between endothelial nitric oxide synthase gene T786C and 4b/a polymorphisms in patients with essential hypertension

To investigate an interaction between eNOS gene T786C and 4b/a polymorphisms in the risk for essential hypertension (EH). Polymerase chain reaction (PCR)combined with restriction enzyme digestion was used to detect the T786C and 4b/a polymorphisms of eNOS gene in 189 normotensive controls and 310 hypertensive patients. The frequencies of a allele, ab+aa genotype and C allele , CC+CT genotype in EH were significantly higher than in control group respectively;The odds ratio(OR) estimated by combined analysis with eNOS 786C and a allele (2.51) was markedly increased compared with that estimated alone from either eNOS 786C (0.542) or a allele(0.389). eNOS 786C and a allele should be a risk factor for EH in chinese Han nationality;there was a significant synergistic effects of eNOS 786C and a allele in EH.