2012Applied Mechanics and MaterialsOpen access

Screening and Characterization of a Bacteria Strain L0 for Degradation of Cypermethrin

Bin Jiang, Jiang Li, Zhiming Li, Kai Kang, Ruo Nan Li

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Abstract

After initial screening on enrichment media, with subsequent sequential isolation on minimal media containing the cypermethrin as sole C source, three degrading strains were isolated from cypermethrin polluted soil. Gas chromatography was used to measure the rates of disappearance of the cypermethrin, the strain L0 was identified as Bacillius sp. preliminarily. The optimum growth conditions for the aerobic degradation of cypermethrin by L0 were 26°C, pH 7. The degrade rate of cypermethrin by L0 is more than 87.54% above conditions after cultivated for 72 hours. Strain L0 is a high effect degradation bacterium should be used for a bioremediation process of the contaminated soils by insecticides and decrease the environmental pollution.

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What this paper is about

After initial screening on enrichment media, with subsequent sequential isolation on minimal media containing the cypermethrin as sole C source, three degrading strains were isolated from cypermethrin polluted soil. Gas chromatography was used to measure the rates of disappearance of the cypermethrin, the strain L0 was identified as Bacillius sp. preliminarily. The optimum growth conditions for the aerobic degradation of cypermethrin by L0 were 26°C, pH 7. The degrade rate of cypermethrin by L0 is more than 87.54% above conditions after cultivated for 72 hours. Strain L0 is a high effect degradation bacterium should be used for a bioremediation process of the contaminated soils by insecticides and decrease the environmental pollution.

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Available abstract

After initial screening on enrichment media, with subsequent sequential isolation on minimal media containing the cypermethrin as sole C source, three degrading strains were isolated from cypermethrin polluted soil. Gas chromatography was used to measure the rates of disappearance of the cypermethrin, the strain L0 was identified as Bacillius sp. preliminarily. The optimum growth conditions for the aerobic degradation of cypermethrin by L0 were 26°C, pH 7. The degrade rate of cypermethrin by L0 is more than 87.54% above conditions after cultivated for 72 hours. Strain L0 is a high effect degradation bacterium should be used for a bioremediation process of the contaminated soils by insecticides and decrease the environmental pollution.

Key concepts: Cypermethrin, Bioremediation, Strain (injury), Degradation (telecommunications), Bacteria, Isolation (microbiology), Chemistry, Biology

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