1991•Journal of Bone and Mineral ResearchRequires access

Bone morphogenetic protein-2b stimulation of growth and osteogenic phenotypes in rat osteoblast-like cells: Comparison with TGF-β1

Theresa L. Chen, Rebecca L. Bates, Andrew T. Dudley, R. Glenn Hammonds, Edward P. Amento

Abstract

The biologic effects of recombinant human bone morphogenetic protein-2b (BMP-2b = BMP-4) were studied and compared with transforming growth factor-beta 1 (TGF-beta 1) in fetal rat osteoblast-like (ROB) cells. Similar to the effects of TGF-beta 1, BMP-2b stimulated DNA and collagen synthesis as well as protein accumulation. Unlike TGF-beta 1, which inhibited alkaline phosphatase activity, BMP-2b enhanced enzyme activity eight-to ninefold over the control level. The present study demonstrates direct actions of BMP-2b on bone-associated cells to stimulate osteogenic phenotypes in vitro and provides a cellular mechanism for the induction of bone formation by BMP-2b in vivo.

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Key concepts: Bone morphogenetic protein, Osteoblast, Alkaline phosphatase, Bone morphogenetic protein 2, Transforming growth factor, Bone morphogenetic protein 7, Cell biology, Stimulation