2010•Journal of Fisheries and Aquatic ScienceRequires access

Effect of Diazinon on Catalase Antioxidant Enzyme Activity in Liver Tissue of Rutilus rutilus

Vida Keramati, Shahla Jamili, M. Ramin

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Abstract

Diazinon on as an organophosphate toxin applied widely in agricultural gardens of Iran, so every year huge volume of diazinon on enter to aquatic ecosystems. In this study, antioxidant enzyme activity (Catalase) were assessed in liver of Rutilus rutilus weighing about 7.0+1.0 g following exposure of fish to 20, 40 and 60 lig L-1 concentration of diazinon at 22.0+1.0°C and acceptable water quality conditions. At the first, LC„ 96 h was determined 4.5 ppm. We have 3 treatments with 3 replicate and 1 controls (without diazinon). on). In each aquarium (Volume: 8 L) were 10 fishes. The result of measuring catalase activity in liver with special kit did not show significant differences between treatment groups and control (p>0.05). Also, regression curve did not show significant correlation between increasing toxin concentration and catalase activity changes. The results showed that in same concentration, catalase activity in time 24 h significantly was higher than times 48 and 96 h (p<0.05). Finally, the results did not show special relationship between different concentrations of diazinon on and catalase activity in fish liver, that is maybe was result of parameters such as antioxidant enzymes conjugative operation, enzymes professional reaction against special pollutant, rapid reactions and acute phase reaction and antioxidant adaptation again. st pollution and antioxidative reaction outbreak in target organ.

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What this paper is about

Diazinon on as an organophosphate toxin applied widely in agricultural gardens of Iran, so every year huge volume of diazinon on enter to aquatic ecosystems. In this study, antioxidant enzyme activity (Catalase) were assessed in liver of Rutilus rutilus weighing about 7.0+1.0 g following exposure of fish to 20, 40 and 60 lig L-1 concentration of diazinon at 22.0+1.0°C and acceptable water quality conditions. At the first, LC„ 96 h was determined 4.5 ppm. We have 3 treatments with 3 replicate and 1 controls (without diazinon). on). In each aquarium (Volume: 8 L) were 10 fishes. The result of measuring catalase activity in liver with special kit did not show significant differences between treatment groups and control (p>0.05). Also, regression curve did not show significant correlation between increasing toxin concentration and catalase activity changes. The results showed that in same concentration, catalase activity in time 24 h significantly was higher than times 48 and 96 h (p<0.05). Finally, the results did not show special relationship between different concentrations of diazinon on and catalase activity in fish liver, that is maybe was result of parameters such as antioxidant enzymes conjugative operation, enzymes professional reaction against special pollutant, rapid reactions and acute phase reaction and antioxidant adaptation again. st pollution and antioxidative reaction outbreak in target organ.

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Available abstract

Diazinon on as an organophosphate toxin applied widely in agricultural gardens of Iran, so every year huge volume of diazinon on enter to aquatic ecosystems. In this study, antioxidant enzyme activity (Catalase) were assessed in liver of Rutilus rutilus weighing about 7.0+1.0 g following exposure of fish to 20, 40 and 60 lig L-1 concentration of diazinon at 22.0+1.0°C and acceptable water quality conditions. At the first, LC„ 96 h was determined 4.5 ppm. We have 3 treatments with 3 replicate and 1 controls (without diazinon). on). In each aquarium (Volume: 8 L) were 10 fishes. The result of measuring catalase activity in liver with special kit did not show significant differences between treatment groups and control (p>0.05). Also, regression curve did not show significant correlation between increasing toxin concentration and catalase activity changes. The results showed that in same concentration, catalase activity in time 24 h significantly was higher than times 48 and 96 h (p<0.05). Finally, the results did not show special relationship between different concentrations of diazinon on and catalase activity in fish liver, that is maybe was result of parameters such as antioxidant enzymes conjugative operation, enzymes professional reaction against special pollutant, rapid reactions and acute phase reaction and antioxidant adaptation again. st pollution and antioxidative reaction outbreak in target organ.

Key concepts: Rutilus, Catalase, Diazinon, Antioxidant, Enzyme, Biology, Biochemistry, Chemistry

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Effect of Diazinon on Catalase Antioxidant Enzyme Activity in Liver Tissue of Rutilus rutilus — Research Paper | ScholarLens