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An Automated Fluorometric Method for Determination of Lactic Dehydrogenase in Serum

Lewis Brooks, Harry G. Olken

Abstract

Abstract A simple automated method for the determination of lactic dehydrogenase (LDH) activity in serum by the forward lactate → pyruvate reaction is presented. Enzyme activity is determined by measuring the fluorescence of DPNH formed after a fixed incubation time. Detailed studies of various parameters of the reaction, reproducibility, and recovery data, and a comparison with two manual methods for LDH are presented.

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Available abstract

Key concepts: Lactic dehydrogenase, L-Lactate dehydrogenase, Lactate dehydrogenase, Chromatography, Chemistry, Lactic acid, Reproducibility, Automated method