T regulatory cells in exacerbation control of chronic obstructive pulmonary disease of mild and moderate stage

Aim: to investigate the levels of peripheral CD4(+)CD25(++) regulatory T cells and the inflammatory and anti inflammatory cytokines in exacerbation dynamics of mild and moderate stage of chronic obstructive pulmonary disease (AECOPD) patients. Methods: Peripheral blood samples were collected from 20 patients with mild AECOPD and 21 patients with moderate AECOPD, aged (55,6±3,2; 51±3 respectively) and 20 man from control group. Lymphocytes were isolated by two-color labeled monoclonal antibodies flow cytometry to examine the quantities and percentage of CD4+CD25+ T cell, CD4+CD25++ (CD4(+)Treg), CD3+CD19- T cell, CD3+CD4+ T cell, CD3+CD8+ T cell, respectively. Enzyme immunodetection was used to detect the expression of interleukin-10 (IL-10), tumor necrosis factor alpha (TNF-alpha), procalcitonin (PCT) and C-reactive protein (CRP). Mann-Whitney test was used for comparison between data before and after treatment and control group. Results. In patients with mild and moderate AECOPD take place significant decreasing of Tregulatory cells (CD4+CD25++) from 3,3±0,8% in control to 1,13±0,17% and 1,3±0,12%%, respectively (p