1964•Clinical ChemistryRequires access

Automated Determination of Serum Alkaline Phosphatase Using a Modified Bodansky Technic

H Keay, J. A. Trew

Abstract

Abstract An automated procedure for the measurement of alkaline phosphatase activity by a modification of the Bodansky method is described. It has been possible to adapt the Fiske-SubbaRow method for phosphate so that alkaline phosphatase is determined by a second run, immediately following the determination of inorganic phosphate. Studies of the effect of time, pH, and concentration of barbital on the enzyme activity are discussed, and the advantages of the method are listed.

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Available abstract

Key concepts: Alkaline phosphatase, Barbital, Inorganic phosphate, Phosphate, Chemistry, Chromatography, Automated method, Phosphatase