Pemurnian Parsial dan Karakterisasi Enzim Kitinase dari Beauveria bassiana

NUNI LAWATI. Partial Purification and Characterization of Chitinase Enzyme from Beauveria bassiana. Supervised by EMAN KUSTAMAN and YADI SURYADI. Beauveria bassiana si an entomopathogenic fungus that produces chitinase enzyme when infecting its host. The purpose of this study was to perform partial purification and characterization of chitinase enzyme from Beauveria bassiana. The result activity chitinase enzyme of qualitative assay shown that Beauveria bassiana produced ekstracelluler chitinase enzyme with chitinolytic index of 1.035. Purification with ammonium sulfate precipitation produce purity level by 1.2 times than the crude enzyme extract and purification with dialisis techniques can increased the purity of enzim by 1.9 times than the crude enzyme extract. The result of characterization assays shown that chitinase enzyme had an optimum activity of pH 4, temperature 50 o C, incubation time 90 minutes, the addition of metal ions Mn 2 + served as activators, and chitinase enzyme from Beauveria bassiana had Km of 0.225 mg/L and Vmax of 0.069 mg/L sec. Chitinase enzyme from Beauveria bassiana had a molecular weight at 60.255 kDa. Key word : Beauveria bassiana, chitinase, partial purification, and characterization of the enzyme PEMURNIAN PARSIAL DAN KARAKTERISASI ENZIM KITINASE DARI Beauveria bassiana